The “Immediate Drop on Demand Technology” (I-DOT) uses a patented non-contact, pressure-based dispensing technology. By applying a well-defined pressure pulse on top of a source microliter plate with holes in the bottom of each well, a droplet is formed and a highly precise and accurate nanoliter droplet is released into any target plate (SBS-compatible format). Larger volumes are achieved by applying up to 400 pulses per second. The I-DOT is a new approach for nanoliter to microliter liquid-handling tasks. The I-DOT One is specifically built for the use with 96 well I-DOT silica plates and I-DOT pure plates, where capillary forces keep the sample liquid in the cavity.